Fig. 5

Interaction effects and response surface analysis. (A) Two-dimensional interaction plot for pH and Erythrosin B concentration. (B) Two-dimensional interaction plot for Erythrosin B concentration and buffer volume. (C) Three-dimensional response surface plot showing the combined effect of pH and Erythrosin B concentration on F0/F. (D) Three-dimensional response surface plot illustrating the interaction between Erythrosin B concentration and buffer volume.