Table 3 Validation parameters for the developed spectrofluorimetric method according to ICH Q2(R2) guidelines.

From: Sustainable spectrofluorimetric determination of berberine in dietary supplements via Erythrosin B Ion-Pair complexation with mechanistic investigation, Box-Behnken optimization, and green chemistry assessment

Parameters

Berberine

Excitation wavelength (nm)

530

Emission wavelength (nm)

555

Linearity range (µg/mL)

0.1-3.0

Slope

1.0829

Intercept

0.9677

Correlation coefficient (r2)

0.9997

LOD (µg/mL)

0.032

LOQ (µg/mL)

0.096

Accuracy (%R) a

99.83 ± 0.982

Repeatability precision (%RSD) b

0.984

Intermediate precision (%RSD) c

1.318

Robustness (%R)

Buffer (pH)

99.76 ± 0.834

Reagent Conc. (µg/mL)

99.59 ± 1.613

Reaction time (min)

101.77 ± 0.811

  1. a Average of 9 determinations (3 concentrations repeated 3 times).
  2. b % RSD of 9 determinations (3 concentrations repeated 3 times) measured on the same day.
  3. c % RSD of 9 determinations (3 concentrations repeated 3 times) measured in the three consecutive days.
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