Fig. 5 | Scientific Reports

Fig. 5

From: Identification of RBX1 as a regulator of LIPT1 transcription and its role in copper-induced cell death in GBM cells

Fig. 5

The results of the signal conduction analysis of the RBX1/GTF2B/LIPT1 axis. (A) Protein expression of RBX1, GTF2B and LIPT1 in astrocyte HA1800 and GBM cell lines detected by WB (n = 3, two-way ANOVA). (B) Impact of RBX1 overexpression or GTF2B knockdown on RBX1 and GTF2B protein expression in GBM cells assessed by WB (n = 3, two-way ANOVA). (C) Effects of RBX1 overexpression or GTF2B knockdown on LIPT1 mRNA expression in GBM cells analyzed by RT-qPCR (n = 3, two-way ANOVA). (D) Effects of RBX1 overexpression or GTF2B knockdown on LIPT1 mRNA expression in GBM cells analyzed by WB (n = 3, two-way ANOVA). (E) In vitro ubiquitination assay to study RBX1-mediated ubiquitination of GTF2B protein (n = 3). (F) Enrichment of GTF2B on the LIPT1 promoter affected by RBX1 overexpression or GTF2B knockdown assessed by ChIP-qPCR (n = 3, two-way ANOVA). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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