Fig. 8
From: Development of a paediatric model of diffuse traumatic brain injury in ferrets
Analysis for IBA-1-positive cells in key white and grey matter regions following injury. Representative images from the corpus callosum (A) and hypothalamus (B) with insets to show typical morphology, with thickening and shortening of microglial branches with injury following creation of binary images from thresholding. Generation of heat-maps shows that IBA1 + density was seen particularly within the white matter and regions directly under the impact (C). Quantitative analysis (D) shows significant increases at 72 h post-injury in the corpus callosum, fornix, septum and hypothalamus. Data was analysed using two-way MANOVA, with Tukey’s corrections for each region of interest. ****p < 0.001, *p < 0.05 compared to 72 h shams, ####p < 0.001, #p < 0.05 compared to 24 h TBI ferrets. Graphs are presented as mean. n = 5–6 per group for 24 h ferrets, n = 10 per group for 3 day-ferrets.
