Table 2 Primary and secondary antibodies used within the study. Antibodies were stripped between round 1 and 2.
From: Development of a paediatric model of diffuse traumatic brain injury in ferrets
Primary Antibody | Secondary antibody | Wavelength |
|---|---|---|
Neurofilament DG Sensor (NFL; MCA-6H63; 1:4000, Biosensis, cat# M-2122-50; RRID: AB_2923484) | Goat Anti-mouse Alexa fluor 555 (Thermo Fisher, Cat# A-21422, RRID: AB_2535844) | Round 1 555 nm |
IBA-1 (Ionized calcium-binding adapter molecule 1; 1:2000, Wako cat# 019–19741, RRID: AB_839504) | Goat Anti-rabbit Alexa fluor 647 (Thermo Fisher, Cat# A-21244, RRID: AB_2535812) | Round 1 647 nm |
APP (amyloid precursor protein; 1:2000, Thermo Fisher, cat# 51–2700, RRID: AB_2533902) | Goat Anti-rabbit Alexa fluor 647 (Thermo Fisher, Cat# A-21244, RRID: AB_2535812) | Round 2 647 nm |
Tomato Lectin (Lycopersicon esculentum; 1:1000, Vector Laboratories cat# B-1175, RRID: AB_2315475) | Streptavidin Alexa fluor 488 conjugate (Thermo Fisher, Cat# S11223, No RRID) | Round 2 488 nm |
