Fig. 4

A Live image of the A. dispar embryos after exposure to UV. The embryos were exposed to 25, 50 or 100 mJ/cm2 UVC at 4dpf and imaged after 24 h post exposure with focusing on fluoroleucophores (yellow or white) on the surface of the yolk. a-d, WT, e-h. gch-/-, i-l gch-/-tyr-/-. Scale bar: 100 μm. B Effects of UV exposure on the distance between fluoroleucophores in the three lines of A. dispar WT, gch-/- and gch-/-tyr-/- after 24 h of UV exposure. Data are presented as mean ± SEM (n = 3). Post-hoc comparisons revealed a significant reduction in cell distances with increasing UV doses between control and UV-treated groups among lines (p < 0.001). WT exhibited the highest overall cell distances, followed by gch-/- and gch-/-tyr-/-.