Fig. 3
From: Interaction of pulsed low frequency electromagnetic field (PEMF) with mitochondria
Effect of NO and PEMF on the respiration activity of mitochondria. (A) Experimental design and a typical experiment with muscle homogenates treated with an NO-donor, releasing 25 µM of NO over 10 min. (B, C) Effect of NO and PEMF in muscle homogenate. (D, E) Effect of NO and PEMF in liver homogenate. State 3 mitochondrial respiration was stimulated by 10 mM glutamate/malate and 2 mM ADP. During the recovery of State 3 respiration, we determined the minimal slope (full inhibition by NO) and maximal slope (highest recovery rate). Conclusion: The data suggests that PEMF does not facilitate recovery following NO-induced inhibition of liver mitochondria either in muscle or in liver homogenates. PEMF – pulse electromagnetic field; NO – nitric oxide. Statistics: The data were analyzed by one-way ANOVA followed by Holm-Sidak’s multiple comparisons test. The data are presented as mean ± SEM (error bars), experimental points (n=) are shown in the corresponding bars. ** - p < =0.01; **** p < 0.0001.
