Abstract
Pakhoi is a traditional fermented beverage consumed by the Jaunsari community of Uttarakhand, India. To ferment Pakhoi a starter culture rich in ethnomedicinal plants named Keem is used. Despite the cultural significance and traditional health claims associated with Pakhoi, scientific evidence regarding its chemical and nutritional composition remains limited. In this study, Pakhoi samples collected from three sites within the Chakrata region were analysed to evaluate their metabolomic profile, nutritional composition, antioxidant capacity, and antimicrobial activity. LC-QTOF-MS analysis revealed variation in bioactive compounds among sampling sites, reflecting differences in local ingredients and fermentation practices. The main metabolites detected could be 6-gingerol, caffeine, capsaicin, camptothecin, caffeyl alcohol, butin and carteolol. The sample with the most metabolites richness was also assessed concerning physicochemical and nutritional parameters. Pakhoi was found to have an acidic pH (3.01 ± 0.02), high carbohydrate and significant crude protein levels. Phytochemical revealed that the total phenolic (7.05 mg GAE mL-1) and flavonoid content (56.85 mg rutin equivalents mL-1) is high and is associated with high antioxidant activity (DPPH: 46.34 ± 0.01%; ABTS: 41.45 ± 0.07; FRAP: 65.38 mM Fe equivalents). Pakhoi is also inhibitory in food-borne pathogens such as Escherichia coli, Salmonella typhi, Shigella sonnei and Vibrio cholerae. In general, the results indicate that Pakhoi is a valuable nutritionally rich phytochemically diverse fermented drink with a strong antioxidant and antimicrobial potential, which proves its traditional use and suggests its potential as a functional fermented beverage.
Data availability
All data generated or analyzed during this study are included in this article.
References
Rawat, J. M., Pandey, S., Debbarma, P. & Rawat, B. Preparation of alcoholic beverages by tribal communities in the Indian Himalayan region. Front. Sustain. Food Syst. 5, 672411. https://doi.org/10.3389/fsufs.2021.672411 (2021).
Bryant, C., Szejda, K., Parekh, N., Deshpande, V. & Tse, B. A survey of consumer perceptions of plant-based and clean meat in the USA, India, and China. Front. Sustain. Food Syst. 3, 11. https://doi.org/10.3389/fsufs.2019.00011 (2019).
Alae-Carew, C. et al. D. The role of plant-based alternative foods in sustainable and healthy food systems: consumption trends in the UK. Sci. Total Environ. 151041 https://doi.org/10.1016/j.scitotenv.2021.151041 (2022).
Nkhata, S. G., Ayua, E., Kamau, E. H. & Shingiro, J. B. Fermentation and germination improve nutritional value of cereals and legumes. Food Sci. Nutr. 6, 2446–2458 (2018).
Xiang, H., Sun-Waterhouse, D., Waterhouse, G. I., Cui, C. & Ruan, Z. Fermentation-enabled wellness foods: a fresh perspective. Food Sci. Hum. Wellness. 8, 203–243 (2019).
Sharma, R., Garg, P., Kumar, P., Bhatia, S. K. & Kulshrestha, S. Microbial fermentation and its role in quality improvement of fermented foods. Fermentation 6, 106. https://doi.org/10.3390/fermentation6040106 (2020).
Zhao, P., Wang, J., Zhao, W., Ma, X. & Sun, H. Antifatigue and anti-aging effects of Chinese rice wine in mice. Food Sci. Nutr. 6, 2386–2394. https://doi.org/10.1002/fsn3.830 (2018).
Wang, F. et al. Effect of Chinese rice wine on protease activity, degradation, and quality characteristics in fish. J. Aquat. Food Prod. Technol. 29, 494–506. https://doi.org/10.1080/10498850.2020.17 (2020).
Haghipanah, M. et al. Investigation of the neuroprotective action of Japanese sake yeast on dementia type of alzheimer disease in rats. NeuroSci 4, 45–53. https://doi.org/10.3390/neurosci4010006 (2023).
Amoutzopoulos, B. et al. Effects of a traditional fermented grape-based drink ‘Hardaliye’ on antioxidant status of healthy adults: a randomized controlled clinical trial. J. Sci. Food Agric. 93, 3604–3610. https://doi.org/10.1002/jsfa.6158 (2013).
Castaldo, L. et al. Red wine consumption and cardiovascular health. Molecules 24, 3626. https://doi.org/10.3390/molecules24193626 (2019).
Marcos, A. et al. Moderate consumption of beer and its effects on cardiovascular and metabolic health: an updated review. Nutrients 13, 879. https://doi.org/10.3390/nu13030879 (2021).
Pires, E. J., Teixeira, J. A., Brányik, T. & Vicente, A. A. Yeast: the soul of beer’s aroma – a review. Appl. Microbiol. Biotechnol. 98, 1937–1949. https://doi.org/10.1007/s00253-013-5470-0 (2014).
Pradhan, P. & Tamang, J. P. Identification of bacteria from dry starters of Eastern Himalayas. Front. Microbiol. 10, 2526. https://doi.org/10.3389/fmicb.2019.02526 (2019).
Majumder, S., Chakraborty, S., Ghosh, A. & Bhattacharya, M. The Himalayan ethnic beverage Tongba with therapeutic properties in high-altitude illnesses and metabolomic similarities to Japanese sake. Acta Univ. Sapientiae Aliment. 15, 67–83. https://doi.org/10.2478/ausal-2022-0006 (2022).
Tamang, J. P., Sarkar, P. K. & Hesseltine, C. W. Traditional fermented foods and beverages of Darjeeling and Sikkim—a review. J. Sci. Food Agric. 44, 375–385. https://doi.org/10.1002/jsfa.2740440410 (1988).
Angmo, K. & Bhalla, T. C. Preparation of Phabs—an Indigenous starter culture for production of traditional alcoholic beverage, Chhang, in Ladakh. Indian J. Tradit Knowl. 13, 347–351 (2014).
Sekar, S. & Mariappan, M. Usage of traditional fermented products by Indian rural folks and IPR. Indian J. Tradit Knowl. 6, 111–120 (2007).
Kishor, L. M., Tewari, A., Tewari, A. & Upreti, B. M. Traditionally fermented alcoholic beverages of high altitude Bhotiya tribal community in Kumaun Himalaya, India. Afr. J. Food Sci. 7, 508–510 (2013).
Rana, T. S., Datt, B. & Rao, R. R. Soor: A traditional alcoholic beverage in tons Valley, Garhwal himalaya. Indian J. Traditional Knowl. 3 (1), 59–65 (2004).
Tomar, S., Pant, K., Sharma, P., Sinha, S. & Mitra, D. Unravelling the hidden ethnic fermented treasure of the Himalayas: A review on the traditionally fermented beverages of the Northwest Indian Himalayan region. Food Chemistry Advances, 2, 100254. (2023). https://doi.org/10.1016/j.focha2023.100254AOAC International. Official Methods of Analysis of AOAC International, 20th ed., 3172 (AOAC International, 2016).
Want, E. J. et al. Global metabolic profiling procedures for urine using UPLC-MS. Nat. Protoc. 5 (6), 1005–1018. https://doi.org/10.1038/nprot.2010.50 (2010).
Dunn, W. et al. Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. Nat. Protoc. 6, 1060–1083. https://doi.org/10.1038/nprot.2011.335 (2011).
Smith, C. A. et al. Siuzdak, Gary. METLIN: A Metabolite Mass Spectral Database. Therapeutic Drug Monitoring 27(6):p 747–751, December 2005. | https://doi.org/10.1097/01.ftd.0000179845.53213.39
Das, B. K. et al. Phytochemical screening and evaluation of analgesic activity of oroxylum indicum. Indian J. Pharm. Sci. 76, 571–575 (2014).
Santhi, S. & Sengottuvel, R. Qualitative and quantitative phytochemical analysis of Moringa concanensis Nimmo. Int. J. Curr. Microbiol. Appl. Sci. 5, 633–640 (2016).
Anand, J. et al. Biochemical characterization and pharmacognostic evaluation of purified catechins in green tea (Camellia sinensis) cultivars of India. 3 Biotech. 5, 285–294. https://doi.org/10.1007/s13205-014-0230-0 (2015).
Khan, F. A. et al. Phytochemical screening of some Pakistanian medicinal plants. Middle East. J. Sci. Res. 8, 575–578 (2011).
Vinci, G., D’Ascenzo, F., Maddaloni, L., Prencipe, S. A. & Tiradritti, M. The influence of tea infusion parameters on polyphenol content and antioxidant activity. Beverages 8, 18. https://doi.org/10.3390/beverages8020018 (2022).
Azzahra, L. F., Fouzia, H., Mohammed, L. & Noureddine, B. Antioxidant response of camellia sinensis and Rosmarinus officinalis aqueous extracts toward H₂O₂ stressed mice. J. Appl. Pharm. Sci. 2, 70–76 (2012).
Dudonné, S., Vitrac, X., Coutière, P., Woillez, M. & Mérillon, J. M. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J. Agric. Food Chem. 57, 1768–1774 (2009).
Payne, A. C., Mazzer, A., Clarkson, G. J. & Taylor, G. Antioxidant assays – findings from FRAP and ORAC in spinach, watercress and rocket leaves. Food Sci. Nutr. 1, 439–444. https://doi.org/10.1002/fsn3.71 (2013).
Mitrevska, K., Grigorakis, S., Loupassaki, S. & Calokerinos, A. C. Antioxidant activity and polyphenolic content of North Macedonian wines. Appl. Sci. 10, 2010. https://doi.org/10.3390/app10062010 (2020).
Yu, X., Zhao, M., Liu, F., Zeng, S. & Hu, J. Antioxidants in volatile Maillard reaction products: identification and interaction. LWT Food Sci. Technol. 53, 22–28 (2013).
Yan, F., Yu, X. & Jing, Y. Chitooligosaccharide-glycine Maillard reaction products: Preparation and antioxidant activity. J. Food Sci. Technol. 55, 712–720. https://doi.org/10.1007/s13197-017-2982-0 (2018).
Ginovyan, M., Petrosyan, M. & Trchounian, A. Antimicrobial activity of some plant materials used in Armenian traditional medicine. BMC Complement. Altern. Med. 17, 50. https://doi.org/10.1186/s12906-017-1573-y (2017).
Tomar, S. et al. Microbial diversity and functional potential of keem: A traditional starter culture for alcoholic beverage—application of next-generation amplicon and shotgun metagenome sequences. Mol. Biotechnol. https://doi.org/10.1007/s12033-023-00839-3 (2023).
Zhao, Y. S. et al. Fermentation affects the antioxidant activity of Plant-Based food material through the release and production of bioactive components. Antioxid. (Basel Switzerland). 10 (12), 2004. https://doi.org/10.3390/antiox10122004 (2021).
Hou, X. et al. Effect of winemaking on phenolic compounds and antioxidant activities of Msalais wine. Molecules 28, 1250. https://doi.org/10.3390/molecules28031250 (2023).
Davoodi, M. et al. Beneficial effects of Japanese sake yeast supplement on biochemical, antioxidant, and anti-inflammatory factors in streptozotocin-induced diabetic rats. Diabetes Metab. Syndr. Obes. 12, 1667–1673. https://doi.org/10.2147/DMSO.S2201 (2019).
Lee, S. H. et al. Effect of fermentation on antioxidant, antimicrobial, anti-inflammatory, and anti-Helicobacter pylori adhesion activity of ulmus Davidiana var. Japonica root bark. Food Sci. Biotechnol. 32 (9), 1257–1268. https://doi.org/10.1007/s10068-023-01259-4 (2023).
Rawangkan, A. et al. Antimicrobial properties of coffee beans and by-products. Front. Nutr. 9, 865684. https://doi.org/10.3389/fnut.2022.865684 (2022).
Hayrunisa, I. et al. Microbiology and antimicrobial effects of kombucha: a short overview. Food Biosci. 56, 103270. https://doi.org/10.1016/j.fbio.2023.103270 (2023).
Zokaityte, E. et al. Antimicrobial potential of fermented milk permeate beverages. Beverages 6, 65. https://doi.org/10.3390/beverages6040065 (2020).
Acknowledgements
The authors express gratitude to the Departments of Biosciences and Microbiology, Graphic Era (Deemed to be University), Dehradun where majority of the lab work was done.
Funding
This work was supported by a grant from Uttarakhand Council of Science and Technology for the project id: UCS&T/R&D-01/19/20/17530. We are highly thankful to the council.
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ST- writing, data curation and investigation; JA- validated and analysed the data; KB data curation; KP- conceptualization and validation of the study, review and editing; DM and AA -review and editing.
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Tomar, S., Pant, K., Anand, J. et al. Nutritional and phytochemical properties of Pakhoi a traditional fermented beverage from Uttarakhand, India. Sci Rep (2026). https://doi.org/10.1038/s41598-026-37533-8
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DOI: https://doi.org/10.1038/s41598-026-37533-8
