Table 1 Kinetic parameters of the free and immobilized LDP for TMB and H2O2.

From: Covalent immobilization of Lepidium draba peroxidase on chitosan-coated magnetic nanoparticles and its application in glucose biosensing

Enzyme form

Km (mM)

Vmax (U.mg−1)

kcat (s−1)

kcat/km (s−1. mM−1)

TMB

H2O2

TMB

H2O2

TMB

H2O2

TMB

H2O2

LDP

0.051

5.5

46.30

107.53

631.14

1111.22

12393.38

201.05

Immobilized LDP

0.029

1.43

96.15

476.20

3938.83

15019.0

136546.18

10513.3

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