Fig. 1

Expression Dynamics and Subcellular Localization of H19 in Pancreatic Cancer. (A) Quantitative analysis of H19 subcellular localization based on LncLocator, indicating its predominant localization in the cytoplasm and exosomes (exosomes: 80%, nucleus: 10%, cytoplasm: 10%). (B) B.Box plot comparison of H19 expression levels in pancreatic cancer tissues (n = 179) and adjacent normal tissues (n = 171) from the TCGA database, *p < 0.05, **p < 0.01, ***p < 0.001. (C) Kaplan-Meier survival curve stratified by H19 expression in the TCGA database. High H19 levels are associated with poor prognosis (hazard ratio = 1.75, 95% confidence interval: 1.10–2.78; p = 0.018). (D) Relative expression of H19 in pancreatic cancer cell lines (PANC-1, MIA PaCa-2, Capan-2, BxPC-3, CFPAC) and normal HPDE cells. The expression of H19 is upregulated by more than 4-fold in PANC-1 and MIA PaCa-2 cells, *p < 0.05, **p < 0.01, ***p < 0.001. (E) Immunofluorescence staining confirmed the enrichment of H19 (red) in the cytoplasm of PANC-1 and MIA PaCa-2.