Fig. 3

Evaluation of oxidative stress and apoptosis in MCF-7 breast cancer cells. TMX: Tamoxifen; NRG: Naringin; DMT: Diosmetin. (A) DCFDA assay showing intracellular ROS generation in cells treated with TMX, NRG, and DMT; (B) AO/EB dual staining illustrating apoptotic morphology in cells treated with the same compounds; (C) DCFDA assay showing ROS generation in combination treatments; (D) AO/EB dual staining depicting apoptosis in combination-treated cells.