Fig. 4

UCMSC-Exos decreased neutrophil counts in the corneal limbus. (A) Representative immunohistochemistry images of the corneal limbus region. CD31 (magenta) indicates blood vessels, and Ly6G (yellow) indicates neutrophils. Scale bars, 10 μm. (B) Quantification of Ly6g⁺ neutrophils was performed on flat-mounted corneas, focusing on the limbus region (n = 5). The total number of corneal neutrophils was counted in nine 40 × fields of view that comprised the diameter of the cornea. (C) Representative flow cytometry plots showing the frequencies of Th17 cells (CD4⁺CD196⁺) and Treg cells (CD4⁺CD25⁺) in eye-draining lymph nodes. (D) Quantification of Th17 cells and Treg cells and the Th17/Treg ratio (n = 5). Data are presented as mean ± SEM for each group (n = 5). Statistical significance is denoted as ^***P < 0.001, ^****P < 0.0001 for the Young group; ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 for the Aged group; and ^{^}P < 0.05, ^{^^^}P < 0.001 for comparisons between the Young and Aged groups. Statistical analyses were performed by one-way ANOVA and Student’s t-test.