Fig. 6

RT-qPCR and Western blot detection of key factors in the PERK/Nrf2/HO-1 pathway. (A-B) mRNA expression levels of Nrf2 and HO-1 in cells from different groups; (C) Western blot analysis of Nrf2, HO-1, PERK, and GAPDH. (D-F) the relative protein expression levels of PERK, Nrf2 and HO-1 in cells from different groups. Data are shown as the mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.n = 3.