Fig. 4
Effects of GALR3 inhibition on retinal inflammation in RhoP23H/+ mice. The analyses were performed in WT, RhoP23H/+, antagonist-treated RhoP23H/+, and RhoP23H/+Galr3−/− mice at postnatal day 33 (P33) and P45. a Detection of GFAP expression (red) (upper panel) and IBA-1 expression (red) (lower panel) in retinal cryosections at P33. Arrows indicate IBA-1-positive immune cells migrating to the outer retina. Detection of GFAP expression (red) (upper panel) and IBA-1 expression (red) (lower panel) in retinal cryosections at P45. Arrows indicate IBA-1-positive immune cells migrating to the outer retina (enlarged images are shown in Fig. S3). Nuclei were stained with DAPI (blue). Scale bar, 50 μm. Detection of GFAP and IBA-1 in retinal cryosections of WT mice is shown in Fig. S4. c Protein expression levels of GFAP in mouse eyes assessed by immunoblotting at P33 and P45. Full immunoblots are shown in Supplementary Information (Fig. S2). d Quantification of protein bands intensities of GFAP detected in three independent immunoblot experiments, normalized to GAPDH. e Detection of IBA-1 positive cells (red) in retinal flat mounts at P33 and P45. f Quantification of IBA-1 positive cells in retinal flat mounts. Error bars represent S.D. Statistical analysis was performed with one-way ANOVA and post hoc Turkey’s tests. Statistically significant changes are shown with asterisks. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns not statistically significant. P23H/+, RhoP23H/+; P23H/+ SNAP, RhoP23H/+ treated with antagonist; P23H/+Galr3−/−, RhoP23H/+Galr3−/−. GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer.
