Fig. 5 | Scientific Reports

Fig. 5

From: CNOT2 /c-Myc/STAT3 signaling is critically involved in glycolysis mediated apoptosis of benzyl isothiocyanate in hepatocellular carcinoma

Fig. 5

Effect of BITC on Warburg effect in SK-Hep1 and Huh7 cells. (A) Effect of BITC on Warburg effect proteins in SK-Hep1 and Huh7 cells. (B) Effect of BITC on LDH production in SK-Hep1 and Huh7 cells. STAT3 or CNOT2 depletion vs untreated control. ***p < 0.001 vs untreated control. (C) Effect of BITC on glucose consumption in SK-Hep1 and Huh7 cells. *p < 0.05, ***p < 0.001 vs untreated control. (D) Effect of pyruvate treatment or overexpression of CNOT2 or c-Myc on apoptosis and glycolysis proteins in BITC treated SK-Hep1 cells. (E) Effect of siSTAT3 or/and siCNOT2 on HK2, PKM2 and LDH in SK-Hep1 cells transfected with or without c-Myc overexpression plasmid. Cells were co-transfected with control vector, siSTAT3, siCNOT2 and siCNOT2 + c-Myc OE (overexpression) plasmids as indicated in each lane. Band intensities were quantified and normalized to β-actin. All experiments were performed using biological triplicates and independently repeated three times.

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