Fig. 2

HG induced L929 fibroblast senescence. (A) The relative proliferation activity of L929 induced by different glucose concentrations was measured by CCK-8 (n = 3). (B) Representative EDU staining images of L929 after 72 h of exposure to different concentrations of glucose (bar scales = 200 μm). (C) Quantitative analysis of EDU staining (n = 3). (D) Representative SA-β-gal staining images of L929 after 72 h of exposure to different concentrations of glucose (bar scales = 500 μm). Results are presented as the mean ± SD, ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.