Fig. 6

aFGF inhibits the senescence of HG-induced cells and tissues through the activation of SIRT1/STAT3 pathway. (A) Western blot images of SIRT1, STAT3 and pSTAT3 expression of diabetic wounds on day 8. (B) Representative images of SA-β-gal staining of L929 treated with EX-527 48 h before staining. (bar scales = 500 μm). (C) The level of ROS in L929 treated with EX-527 48 h before detection (bar scales = 200 μm). (D) Western blot images of p21, p53, SIRT1, STAT3 and pSTAT3 in L929 treated with EX-527 48 h before extracting the total cellular protein.