Fig. 3

Effects of aniracetam on the Levels of 5-HT, 5-HIAA, and DA in PFC microdialysates of TARP γ-8 KO mice (Mean ± SEM, n = 8). Microdialysis was used to monitor extracellular neurotransmitter levels. Data are expressed as mean ± SEM. (D) 5-HT levels. TARP γ-8 KO (vehicle) mice showed significantly lower 5-HT levels compared to WT (vehicle) (P < 0.0001). Aniracetam treatment significantly elevated 5-HT levels in KO mice relative to the TARP γ-8 KO(vehicle) group (P < 0.0001). (E) 5-HIAA levels. 5-HIAA levels were significantly reduced in TARP γ-8 KO (vehicle) mice versus WT (vehicle) (P < 0.0001). Aniracetam administration significantly increased 5-HIAA levels in KO mice (P < 0.0001). (F) DA levels. DA levels were significantly decreased in TARP γ-8 KO (vehicle) mice compared to WT (vehicle) (P < 0.0001, unpaired t-test with Welch’s correction). Aniracetam treatment significantly restored DA levels in KO mice (P < 0.0001, unpaired t-test with Welch’s correction). All data met normality assumption and were analyzed by unpaired t-test (or Welch’s correction where specified). ^####P < 0.0001, ^###P < 0.001, ^##P < 0.01, ^#P < 0.05(TARP γ-8 KO (vehicle) vs. WT (vehicle)); ^****P < 0.0001, ^***P < 0.001, ^**P < 0.01, ^*P < 0.05 (TARP γ-8 KO (aniracetam) vs. TARP γ-8 KO (vehicle)).