Fig. 4

RT-qPCR analysis of mRNA expression of eight Genes in the PFC of TARP γ-8 KO mice (Mean ± SEM, n = 3). To validate the transcriptomic findings, qPCR was performed to measure the mRNA expression levels of eight genes (gria1, gria2, gria3, gria4, slc6a3, slc6a4, slc6a9, and gabra1) in the PFC of mice from the WT (vehicle), TARP γ-8 KO (vehicle), and TARP γ-8 (aniracetam) groups. gria1 expression: TARP γ-8 KO (vehicle) group vs. WT (vehicle) group, P < 0.05; TARP γ-8 (aniracetam) group vs. TARP γ-8 KO (vehicle) group, P < 0.05; gria2 expression: TARP γ-8 KO (vehicle) group vs. WT (vehicle) group, P < 0.01; TARP γ-8 (aniracetam) group vs. TARP γ-8 KO (vehicle) group, P < 0.001; gria3 expression: TARP γ-8 KO (vehicle) group vs. WT (vehicle) group, P < 0.05; TARP γ-8 (aniracetam) group vs. TARP γ-8 KO (vehicle) group, P < 0.05; gria4 expression: TARP γ-8 KO (vehicle) group vs. WT (vehicle) group, P = 0.0744; TARP γ-8 (aniracetam) group vs. TARP γ-8 KO (vehicle) group, P = 0.0536; slc6a3 expression: TARP γ-8 KO (vehicle) group vs. WT (vehicle) group, P < 0.001; TARP γ-8 (aniracetam) group vs. TARP γ-8 KO (vehicle) group, P < 0.0001; slc6a4 expression: TARP γ-8 KO (vehicle) group vs. WT (vehicle) group, P < 0.01; TARP γ-8 (aniracetam) group vs. TARP γ-8 KO (vehicle) group, P < 0.01; slc6a9 expression: TARP γ-8 KO (vehicle) group vs. WT (vehicle) group, P < 0.01; TARP γ-8 (aniracetam) group vs. TARP γ-8 KO (vehicle) group, P < 0.01; gabra1 expression: TARP γ-8 KO (vehicle) group vs. WT (vehicle) group, P < 0.01; TARP γ-8 (aniracetam) group vs. TARP γ-8 KO (vehicle) group, P < 0.01. ^####P < 0.0001, ^###P < 0.001, ^##P < 0.01, ^#P < 0.05 (TARP γ-8 KO (vehicle) vs. WT (vehicle)); ^****P < 0.0001, ^***P < 0.001, ^**P < 0.01, ^*P < 0.05 (TARP γ-8 KO (aniracetam) vs. TARP γ-8 KO (vehicle)).