Fig. 2
Differentially expressed neutrophil miRNAs and impaired apoptotic activation (A) A lollipop plot represents differentially expressed miRNA of non-survivors (n = 4) relative to survivors (n = 2) purified neutrophils. The x-axis represents miRNA ID, and the y-axis represents log2 fold changes. Each lollipop represents a differentially expressed miRNA between the two groups, with green lollipops indicating upregulated miRNAs and red lollipops indicating downregulated miRNAs. The size of the dot represents the significance level of the change in gene expression. (B) Apoptosis pathway map (hsa04210) rendered with Pathview with differential expression overlaid. Pathway map obtained from the Kyoto Encyclopedia of Genes and Genomes61,63. Copyright Kanehisa Laboratories. Reproduced with permission. KEGG apoptosis pathway visualization built from predicted miRNA–target relationships (miRCarta; experimentally supported targets only). Boxes denote genes; fill follows the color scale in the panel to indicate predicted direction/magnitude based on miRNA regulation (note: this diagram reflects predicted targets from miRNA data and is not direct mRNA/protein measurement). (C) Representative cleaved caspase−3 (CC3) immunofluorescence in freshly isolated neutrophils (uniform acquisition parameters). (D) Per-cell CC3 intensity normalized to DAPI (controls; n: 627 cells, N = 5; ICU-S; n: 461 cells, N = 2; ICU-NS; n: 1,178 cells, N = 5). Scale bar, 100 μm. CC3 intensity was reduced in both ICU-S and ICU-NS compared with controls, but did not differ between ICU-S and ICU-NS (p = 0.62). Data are mean ± SD. ANOVA was done followed by Tukey’s post-hoc tests to compare the differences between the three groups.
