Fig. 5
From: Proteomic profiling of equine airway mucus reveals compositional changes in asthmatic phenotypes
Functional enrichment analysis of proteins detected in BALF mucus of SEA and MEA horses. Significantly increased proteins (log2 fold change ≥ 1 or ≤ −1, q-value < 0.05, s0 = 0.1) from SEA and MEA groups were subjected to overrepresentation analysis using Gene Ontology (GO, a-c) and Reactome pathway annotations (d). Displayed are the top ten nonredundant terms or pathways. (a) GO: Biological Process (BP) terms enriched in SEA (red) and MEA (yellow) samples. (b) GO: Cellular Component (CC) terms (c) GO: Molecular Function (MF) terms illustrating functional protein properties. (d) Reactome pathway enrichment. The x-axis indicates - log₁₀ adjusted p-values, the section size is reflected by the sizes of the dots. (e) Gene Set Enrichment Analysis (GSEA) on ranked protein lists from the differential abundance analyses of SEA and MEA relative to healthy controls. Normalized enrichment scores (NES) of selected positively enriched Hallmark gene sets (MSigB v2024.1, h.all.v2024.1.Hs.symbols.gmt) in SEA (red) and MEA (yellow) compared to healthy controls. Displayed are the four significantly enriched pathways across both groups.
