Fig. 3 | Scientific Reports

Fig. 3

From: Bifidobacterium breve promotes growth and lipid alteration in Trichomonas vaginalis transiently through transcriptomic reprogramming

Fig. 3The alternative text for this image may have been generated using AI.

B. breve does not exhibit cytoprotective activity during T. vaginalis infection. (A) The secretions of IL-6 from HeLa cells infected with T. vaginalis and B. breve-treated T. vaginalis were determined after 24 hours of incubation. The treated cells were washed, incubated, stained with Giemsa, and monitored under a microscope: (B) upper left, HeLa cells only; upper right HeLa cells treated with B. breve for 3 hours and cultured in serum- and antibiotic-free DMEM for 12 hours; lower left, HeLa cells were co-cultured with T. vaginalis in serum- and antibiotic-free DMEM for 12 hours; lower right, HeLa cells were pretreated with B. breve in serum- and antibiotic-free DMEM for 3 hours, followed by infection with T. vaginalis under the same conditions for 12 hours. (C) The quantified chart of (B). (D) The same settings as B were applied for heat-killed B. breve (HK-Bb) instead of viable B. breve. (E) The quantified chart of (D). Statistical significance: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Back to article page