Table 1 Summary of characterised CTS mutants and ConSurf analysis.
Residue | Conserved score | Predicted description | Biochemical result | Reference |
|---|---|---|---|---|
D606 | 9 | Exposed Functional (Walker B motif) | D606N in planta doesn’t germinate doesn’t establish | |
D863 | 9 | Exposed Functional | D863A in S. cerevisiae defective of β-oxidation D863A in peroxisomes retained substrate-stimulated ATPase activity | |
E300 | 9 | Exposed Functional | E300D in A.thaliana similar phenotype to WT germination | |
E953 | 9 | Exposed Functional | E395D in A.thaliana were IBA and 2,4-DB sensitive | |
K487 | 9 | Exposed Functional (Walker A motif) | K487A in planta doesn’t germinate doesn’t establish K487A in Sf9 membranes: greatly reduced ATPase (not substrate stimulated) and no ATP-dependent ACOT activity | |
P539 | 9 | Exposed Functional | P539L in planta doesn’t germinate doesn’t establish | |
Q864 | 9 | Exposed Functional | Q864A Sf9 membranes: similar ATPase activity to WT but greatly reduced ACOT activity Q864A in S. cerevisiae defective of β-oxidation Q864A in peroxisomes retained substrate-stimulated ATPase activity | |
S810 | 8 | Buried | S810N in planta germinates(slow) but doesn’t establish S810N S. cerevisiae pxa1/pxa2Δ mutant reduced ACOT and unable to support oleate β-oxidation S810N Sf9 membranes: similar ATPase activity to WT but greatly reduced ACOT activity | |
T867 | 8 | Exposed Functional | T867A in S. cerevisiae defective of β-oxidation T867A in peroxisomes retained substrate-stimulated ATPase activity |
