Fig. 2 | Scientific Reports

Fig. 2

From: Cannabidiol mitigates secondary genital injury after thoracic trauma by regulating systemic inflammation and hormone receptor signaling

Fig. 2

Experimental design of blunt chest trauma and cannabidiol (CBD) treatment in rats. Adult rats were randomly assigned to four groups: Sham, Trauma, Trauma + CBD, and CBD. Blunt chest trauma was induced under anesthesia by dropping a 200 g cylindrical weight from a height of 1 m onto the thoracic wall. CBD (5 mg/kg, i.p.) was administered 30 min prior to trauma in the Trauma + CBD group, and to non-traumatized rats in the CBD group. Forty-eight hours after trauma, animals were euthanized, and genital tissues (ovaries, uterus, fallopian tubes) were collected. Histopathological and immunohistochemical analyses were performed to evaluate estrogen receptor (ER), hypoxia-inducible factor-1α (HIF-1α), and oxytocin receptor (OTR) expression.

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