Fig. 2
From: Role of the ABCG2 transporter in the biodistribution of the food-borne uremic toxin p-cresyl sulfate
Transepithelial transport assay of p-cresyl sulfate (10 µM) in the presence or absence of Ko143 (1 µM), the ABCG2 specific inhibitor, in parental MDCK-II cells (a and b, respectively) and its subclones transduced with murine (mAbcg2) (c and d, respectively), human (hABCG2) (e and f, respectively), ovine (oABCG2) (g and h, respectively) and bovine (bABCG2) (i and j, respectively) variants of the transporter. Initially, medium of both compartments was replaced with fresh culture medium with 10 µM p-cresyl sulfate, containing or not the inhibitor. Aliquots were collected at 1, 2, 3 and 4 h on the opposite side where the potential substrate had been added. All samples were stored at − 20 °C until being analyzed by ultra-performance liquid chromatography. p-Cresyl sulfate detected in the opposite compartment is expressed as the percentage of the total compound added at the beginning of the experiment. (n ≥ 4). Results are shown as mean ± S.D.
