Table 1 Relative efflux transport (BA/AB) ratio, apically directed translocation percentage (%BA transport) divided by basolaterally directed translocation percentage (%AB transport), at 4 h for p-cresyl sulfate (10 µM) in parental MDCK-II cells and its subclones transduced with murine (mAbcg2), human ABCG2 (hABCG2), ovine (oABCG2) and bovine (bABCG2) variants, either in the absence (-Ko143) and the presence of the ABCG2 specific inhibitor Ko143 (1 µM) (n ≥ 4).

From: Role of the ABCG2 transporter in the biodistribution of the food-borne uremic toxin p-cresyl sulfate

 

-Ko143

+Ko143

%BA transport

%AB transport

Ratio BA/AB

%BA transport

%AB transport

Ratio BA/AB

MDCK-II

10.50 ± 2.20

10.18 ± 2.51

1.04 ± 0.10

10.57 ± 2.23

11.03 ± 2.26

0.97 ± 0.10

MDCK-II mAbcg2

17.41 ± 2.50

4.53 ± 0.34

3.83 ± 0.31***

9.03 ± 2.74

9.54 ± 2.61

1.01 ± 0.06#

MDCK-II hABCG2

9.56 ± 2.60

4.22 ± 1.46

2.31 ± 0.28**

5.70 ± 2.70

5.64 ± 2.55

1.00 ± 0.16#

MDCK-II oABCG2

17.84 ± 9.44

1.30 ± 0.82

15.47 ± 4.79**

6.19 ± 2.27

5.99 ± 2.66

1.06 ± 0.13#

MDCK-II bABCG2

14.34 ± 2.24

6.78 ± 2.09

2.21 ± 0.43*

8.68 ± 1.54

9.38 ± 1.67

0.93 ± 0.06#

  1. Results are shown as mean ± S.D. (*) p ≤ 0.05, (**) p ≤ 0.01, and (***) p ≤ 0.001: significant differences in transport ratio compared to parental MDCK-II cells (Student’s t-test, normally distributed data). (#) p ≤ 0.05 significant differences compared to treatment without Ko143 (Student’s t-test, normally distributed data).
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