Table 6 Forward-reverse oligonucleotide-based real-time PCR primers for the immunological and antioxidant genes being studied.
Investigated marker | Primer | Product size (bp) | Annealing temperature (°C) | GenBank isolate | Origin |
|---|---|---|---|---|---|
SLC11A1 | F5′- ACTCGCTCCTGTGCCATCCTG − 3 R5′- CTTCTCCTGATCCTCTTCAGGA-3′ | 429 | 60 | FJ827149.1 | |
CD14 | F5′- CAGTTGCGACATCTGGACCTC − 3 R5′- AGTGTCATATACAATGAAAGCAT − 3′ | 360 | 60 | DQ457089.1 | Present research |
PTX3 | F5′- GACGAGCTGCAGAAGCTGCGG − 3′ R5′- TGCAGGCGCTGAAAGTCTCGAG − 3′ | 388 | 58 | XM_006054085.4 | |
IRF3 | F5′- CCTCGGATACTGCCCTGGCTGA-3′ R5′- TCAAGTCCATGTCACTCAGTAAC − 3′ | 430 | 58 | XM_025270005.3 | |
PRDX2 | F5′- CAAGTTCGTGCATCCGAGAGTC − 3′ R5′- TCATCTTCCTTCAGCACGCCATA- 3′ | 425 | 58 | XM_006041572.4 | |
PRDX6 | F5′- GGAGGTCTCCTCCTCGGGGACG − 3′ R5′- AATCTCATCAAAGTTCCTGCCAG − 3′ | 473 | 58 | MH085038.1 | |
GPX | F5′- TGCGAGGTGAATGGCGAGAAGGC- 3′ R5′- CTAGGCAGAGGCCCCCTGGGACA − 3′ | 267 | 58 | JN801165.1 | |
ST1P1 | F5′- TGAGTGCCGGCAACATTGACGA − 3′ R5′- AGGTCGGACGGCTTGTTCCGCA − 3′ | 454 | 60 | XM_006050858.3 | |
GAPDH | F5′- GGCAAAGTGGACATCGTCGCCA-3′ R5′- AGCCTTCTCCATGGTAGTGAAG − 3′ | 249 | 58 | AY974798.1 |
