Fig. 5 | Scientific Reports

Fig. 5

From: High-intensity interval training remodels perineuronal nets in the medial prefrontal cortex to drive microglial polarization and alleviate osteoarthritis pain

Fig. 5

PNNs degradation and HIIT alleviate neuroinflammation in the mPFC of OA rats. (A-D) Representative triple immunofluorescence staining images of the mPFC, showing the distribution patterns of PNNs, Iba1 (microglial marker), and iNOS (A) or Arg1 (C). Quantification of panel A is shown in (B), and quantification of panel C is shown in (D) (n = 6). (E and F) Western blot analysis and corresponding quantification of inflammatory markers, including iNOS, Arg1, IL-1β, TNF-α, and IL-10 in mPFC tissue lysates (n = 6). Statistical methods used: One-way ANOVA. **p < 0.01 vs. the OA+Vehicle group, ns not significant vs. the OA+ChABC group.Data are presented as mean ± SD.

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