Abstract
It is widely accepted that tinnitus arises from maladaptive neuroplasticity in the central auditory system, which compensates for reduced peripheral input; notably, the loss of inner hair cell (IHC) synapses is considered a key underlying factor. IHC synapses can immediately and permanently degenerate after noise exposure, independent of hair cell loss, whereas the cell bodies and central projections of cochlear neurons can survive for months to years, providing an extended therapeutic window for improving afferent input through synapse regeneration. Using NHPN-1010 (a combination of 2,4-disulfophenyl-N-tert-butylnitrone and N-acetylcysteine), we propose a delayed treatment strategy (≥ 4 weeks after trauma) for noise-induced tinnitus (NIT) in rats. NHPN-1010 enhanced gap-prepulse inhibition of acoustic startle responses—indicative of diminished tinnitus-like deficits—coupled with a concomitant upregulation of GABAergic inhibition in the dorsal cochlear nucleus, relative to vehicle alone (saline). Furthermore, NHPN-1010, compared to saline, led to increased wave-I amplitudes of auditory brainstem responses, the effect accompanied by a corresponding rise in the number of presynaptic ribbons in IHCs. Our findings suggest that NHPN-1010 plays a therapeutic role in NIT by promoting cochlear synaptogenesis and normalizing central auditory signaling.
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This research was supported by The Oklahoma Center for the Advancement of Science and Technology (OCAST) grant # AR20-014.
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J.L., X.D., R.D.K. and M.B.W. designed research; J.L., X.D., Z.Y., Q.C., W.C., D.N., W.L. and X.H. performed research; J.L. analyzed data; J.L., X.D. and M.B.W. wrote the paper.
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Richard D. Kopke has a financial interest in Otologic Pharmaceutics, Inc. All other authors declare no competing interests.
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Lu, J., Du, X., Yokell, Z. et al. A delayed pharmacological treatment strategy attenuates noise-induced tinnitus in rats. Sci Rep (2026). https://doi.org/10.1038/s41598-026-40960-2
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DOI: https://doi.org/10.1038/s41598-026-40960-2


