Fig. 4 | Scientific Reports

Fig. 4

From: Effects of dietary vitamin D supplementation on bone microarchitecture, mineralization, and mechanical properties in Wistar rat animal model

Fig. 4

Effects of dietary vitamin D supplementation on bone plate and metaphyseal morphometry in male and female Wistar rats. Animals were assigned to one of three dietary groups: 0 IU/kg (Group I), 1000 IU/kg (Group II), or 5000 IU/kg (Group III) of vitamin D₃ (n = 6 per sex per group): (a) resting zone [Zone I], (b) proliferation zone [Zone II], (c) hypertrophic zone [Zone III], (d) ossification zone [Zone IV] (e) bone volume to total volume ratio, (f) metaphyseal trabecular number, (g) metaphyseal trabecular thickness and (h) metaphyseal trabecular separation. Data are presented as mean ± SEM. Statistical analyses were conducted in GraphPad Prism (version 10.5.0) using two-way ANOVA to assess the effects of vitamin D dose, sex, and their interaction. Statistical significance was set at p < 0.05. Capital letters indicate significant differences between dietary doses within the sex groups; brackets denote significant differences between sexes; and asterisks (*) represent p-values (**p < 0.01; ***p < 0.001).

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