Fig. 2
(a) Example of activity-dependent slowing with the marking method and additional stimuli. Each line begins with the periodic background pulse indicated by the blue rectangle, resulting in signal segments with a duration of 4 s. When no extra activity is observed (lines 1–3), the spikes (green dots) align vertically due to the almost constant conduction velocity. When additional (electrical) stimuli are applied, there is a decrease in conduction velocity (line 4). Responses to background stimuli are marked by green dots with a blue circle. (b) Example of activity-dependent slowing stimulated with the ground truth protocol. As in the routinely used marking method, there are background stimuli every 4 s and additional pulses as in (a). In contrast to the “normal” marking method, the signal is cut before each stimulation pulse, whether it is a background pulse or an additional pulse. This ensures that all spikes are aligned on the green track and not only the ones evoked by background stimuli. Responses to background stimuli remain marked by green dots with blue circles, while responses to additional stimuli are indicated by purple rectangles and circles, both for the stimuli and the evoked spikes.
