Table 1 Primer sequences used in qRT-PCR.
From: Local ice cryotherapy reduced vascular inflammation in large artery from rats with arthritis
Target | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
|---|---|---|
Arg-2 | CTC-TGG-ATC-TTG-TTG-AAG | ACT-TGA-AGC-AAT-CAC-ATC |
ICAM-1 | TGC-CTG-CAC-TTT-GCC-CTG-GT | ACA-GGC-CCG-GGG-ATC-ACA-AC |
VCAM-1 | TTG-TTC-AAG-AGA-AAC-CAT-TTA-GTG-T | TCA-TCC-TCA-ACA-CCC-ACA-GG |
CXCL-1 | CCA-GCC-ACA-CTC-CAA-CAG-AGC-A | GGC-GCC-CCT-GTG-GCT-TGG- |
CCL-2 (MCP-1) | GTG-TGA-TTT-GGA-ATG-TGA-TG | AAG-TGT-TGA-ACC-AGG-ATT |
CCL-3 (MIP-1α) | AGA-ACA-TTC-CTG-CCA-CCT | AAG-TGA-AGA-GTC-CCT-GGA-T |
COX-2 | TTT-GCC-TCT-TTC-AAT-GTG | TTA-ATG-TCA-TCT-AGT-CTG-GA |
P22 phox | ACC-TGA-CCG-CTG-TGG-TGA-A | GTG-GAG-GAC-AGC-CCG-GA |
P47 phox | TCC-TAT-CCC-TAC-CCT-TGT | GAG-TCT-GAG-TCC-ATT-CCA |
β-Actin | TAT-CGG-CAA-TGA-GCG-GTT-GC | TGC-CTG-GGT-ACA-TGG-TGG-TG |
GAPDH | GGG-CAT-CCT-GGG-CTA-CAC-TG | GAG-GTC-CAC-CAC-CCT-GTT-GC |
