Fig. 7
From: Non-severe burn injury leads to sustained molecular changes in the brain in a murine model
OPLS-DA and spectra figures of the HR-MAS NMR of significant overall differences between intervention groups in the prefrontal cortex and the cerebellum. OPLS-DA scores plot of 1D 1H NMR profiles sham (blue) vs. burn (orange) in the prefrontal cortex (R2X = 0.22, CV-AUROC = 1.00) (A), 1H NMR backscaled coefficients plots from the OPLS-DA model showing significant metabolite peaks (B). OPLS-DA scores plot of 1D 1H NMR profiles sham (blue) vs. excision (red) in the prefrontal cortex (R2X = 0.22, CV-AUROC = 0.90) (C), 1H NMR backscaled coefficients plots from the OPLS-DA model showing significant metabolite peaks (D). Key metabolites in (B) and (D): (1) Lactate; (2) Alanine; (3) 4-aminobutyrate (GABA); (4) Acetate; (5) N-acetylaspartate; (6) Glutamine; (7) Aspartate; (8) Creatine; (9) Choline; (10) Myo-inositol. OPLS-DA scores plot of 1D 1H NMR profiles of sham (blue) vs. burn (orange) in the cerebellum (R2X = 0.10, CV-AUROC = 0.77) (E), 1H NMR backscaled coefficients plots from the OPLS-DA model showing significant metabolite peaks (F). OPLS-DA scores plot of 1D 1H NMR profiles of sham (blue) vs. excision (red) in the cerebellum (R2X = 0.14, CV-AUROC = 0.87) (G), 1H NMR backscaled coefficients plots from the OPLS-DA model showing significant metabolite peaks (H). OPLS-DA scores plot of 1D 1H NMR profiles of burn (orange) vs. excision (red) in the cerebellum (R2X = 0.13, CV-AUROC = 0.64) (I), 1H NMR backscaled coefficients plots from the OPLS-DA model showing significant metabolite peaks (J). Key metabolites in (F, H, and J): (1) Lactate; (2) Alanine; (3) 4-aminobutyrate (GABA); (4) Acetate; (5) N-acetylaspartate; (6) Glutamate; (7) Glutamine; (8) Aspartate; (9) Creatine; (10) Choline; 11. Phosphocholine; 12. Glycerophosphocholine; 13. Taurine; 14. Myo-inositol; 15. Glycine.
