Fig. 8 | Scientific Reports

Fig. 8

From: Ultrasound-guided skeletal muscle biopsy technique permits measurement of structural, functional, cellular and biochemical properties

Fig. 8The alternative text for this image may have been generated using AI.

Additional applications A) Pax7 + satellite cells per 100 fibers in VL and TA samples. (B) RT-PCR gene expression results, normalized to actin-beta, reported as dCq values. (C) Collagen content of VL and TA samples D). Mitochondrial respiration in fresh, permeabilized VL and TA samples. Sequential protocol of Pyruvate, Malate (Leak), ADP (PCId), Glutamate (PCI), Succinate (PCI + II), FCCP (ECI + II), Rotenone (ECII), Antimycin A (ROX) was used to test maximal oxidative phosphorylation and electron transport chain capacity. (2-way mixed effects ANOVA of substrate x muscle showed substrate effect p < 0.0001). Data reported as mean ± SD. A-C were analyzed with unpaired t-tests, α was set to 0.05.

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