Fig. 5
From: PGE2 regulates ferroptosis and osteogenesis of MC3T3-E1 cells via NOS2
Effect of NOS2 knockdown on PGE2-inhibited osteogenic activity. (A,B): The mRNA expressions of Runx2 and Opn. (C,D) The Western blot results of RUNX2 and OPN expressions. (E,F) Results of ALP staining (40×, bar = 500 μm, representative images are shown). (G) Results of relative ALP activity assay (mean ± SD, n = 3 independent experiments) (H,I) Results of ARS staining (40×, bar = 500 μm, representative images are shown). (J) Results of relative Ca2+ level determination (mean ± SD, n = 3 independent experiments). (Original blots are presented in Supplementary Fig. 2) **P < 0.01, ***P < 0.001, ****P < 0.0001.
