Fig. 8
From: PGE2 regulates ferroptosis and osteogenesis of MC3T3-E1 cells via NOS2
Effect of NOS2 overexpression in non-inflammation context on ferroptosis and osteogenic function. (A–C) The mRNA expressions of Acsl4, Fth1 and Gpx4. (D,E) The Western blot results of ACSL4, FTH1 and GPX4 expressions. (F,G) Relative ROS levels assessed by flow cytometry. (H) Relative MDA levels. (I,J) The mRNA expressions of Runx2 and Opn. (K,L) The Western blot results of RUNX2 and OPN expressions. (M,N) Results of ALP staining (40×, bar = 500 μm, representative images are shown). (O) Results of relative ALP activity assay (mean ± SD, n = 3 independent experiments). (P,Q) Results of ARS staining (40×, bar = 500 μm, representative images are shown). (R) Results of relative Ca2+ level determination (mean ± SD, n = 3 independent experiments). (Original blots are presented in Supplementary Fig. 5) *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
