Fig. 1
From: Data-driven design of LNA-blockers for efficient contaminant removal in Ribo-Seq libraries
LNA design and application considerations. (a) Optimal architecture of LNA probes. Alternating DNA and LNA nucleotides, beginning with a DNA nucleotide. The dotted line represents variable length. Phosphate residue at the 3’-end. (b) Blocking performance of a single LNA against its target (fragment of 5.8 S rRNA) at various concentrations. Comparison between blocking at the reverse transcription step and during PCR amplification. Target abundance percentages are based on qPCR cycle difference in relation to the non-depleted sample (0 µM).
