Abstract
Aflatoxin B1 (AFB1) contamination and micronutrient deficiencies pose a major challenge to food safety and nutritional security. This study elucidated the synergistic potential of boron (B) and zinc (Zn) co-application to fortify nutritional quality and mitigate AFB1 accumulation in turnip (Brassica rapa L) roots. Seeds were cultivated in soils amended with individual or combined B and Zn at concentrations of 10–25 mg kg− 1. In results, individual B supplementation at 20 mg kg− 1 optimized protein content (10.9%), and the B-Zn interactome provided superior overall metabolic performance. B-Zn synergy significantly enhanced physiological resilience. Specifically, the combined application at 15–20 mg kg− 1 consistently achieved the highest STI and GMP productivity across biochemical traits. Carbohydrate partitioning was significantly improved, with NFE reaching 74.1% at 20 mg kg− 1 of B + Zn. AFB1 toxicity was suppressed by 60.08% at 15 mg kg− 1. RPI of dry matter, protein, and phenolic were consistently positive at 15–20 mg kg− 1 of B + Zn. Co-application enhanced the YSI for NFE, protein, and ash content. PCA confirmed that the synergistic effects of B + Zn treatment provided superior nutritional results compared to individual micronutrient applications. These findings demonstrate that balanced B-Zn supplementation strengthens nutritional composition quality and suppresses AFB1 contamination, supporting the biofortification paradigm as a reproducible strategy for sustainable food quality and crop improvement.
Data availability
All data generated or analyzed during this study are included in this published article and its supplementary file.
Abbreviations
- AAS:
-
Atomic Absorption Spectrometry
- AFB1 :
-
Aflatoxin B1
- APX:
-
Ascorbate Peroxidase
- AOAC:
-
Association of Official Analytical Chemists
- ACA:
-
Acetyl-CoA Carboxylase
- BOR:
-
Boron-Requiring Efflux Transporter
- B:
-
Boron
- CAT:
-
Catalase
- Cd:
-
Cadmium
- Cr:
-
Chromium
- DW:
-
Dry weight
- ELISA:
-
Enzyme-Linked Immunosorbent Assay
- FAO:
-
Food and Agriculture Organization of the United Nations
- Fe:
-
Iron
- FW:
-
Fresh Weight
- GAE:
-
Gallic Acid Equivalents
- GMP:
-
Geometric Mean Productivity
- GTs:
-
Glycosyltransferases
- HBV:
-
Hepatitis B Virus
- HCV:
-
Hepatitis C Virus
- HCC:
-
Hepatocellular Carcinoma
- HCN:
-
Hydrogen Cyanide
- IBGE:
-
Institute of Biotechnology and Genetic Engineering
- Mg kg− 1 :
-
Milligram per kilogram
- µ kg− 1 :
-
Microgram per kilogram
- Kcal 100 g− 1 :
-
Kilocalories per 100 g
- MTs:
-
Mycotoxins
- n:
-
Number
- Ni:
-
Nickel
- N:
-
Nitrogen
- NFE:
-
Nitrogen-Free Extract
- NIP:
-
Noudlin-26-like Intrinsic Protein
- NRAMP:
-
Natural Resistance-Associated Macrophages Protein
- PCA:
-
Principal Component Analysis
- Pb:
-
Lead
- Ppb:
-
Parts per billion
- POD:
-
Peroxidase/Guaiacol Peroxidase
- ROS:
-
Reactive Oxygen Species
- Rt-qPCR:
-
Reverse Transcription Quantitative Polymerase Chain
- RPI:
-
Relative Performance Index
- SOD:
-
Superoxide Dismutase
- STI:
-
Stress Tolerance Index
- TOL:
-
Tolerance Index
- SUT:
-
Sucrose Transporter gene
- WHO:
-
World Health Organization
- YSI:
-
Yield Stability Index
- ZIP:
-
Zinc Regulated Transporter
- Zn:
-
Zinc
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Funding
This study was financially supported by Project MECESUP UCT 0804.
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Unays Siraj (U.S): Conceptualization, Data curation, Funding acquisition, Formal analysis, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing – original draft. Zainab Siraj (Z.S): Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing. Patricio R. De Lo Rios-Escalante (P.D.L.R.E): Writing – review & editing, Funding acquisition.
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Siraj, U., Siraj, Z. & De Lo Ríos-Escalante, P.R. Synergistically micronutrient co-application improves nutritional quality and effectively reduces aflatoxin contamination in Brassica rapa L. roots. Sci Rep (2026). https://doi.org/10.1038/s41598-026-43201-8
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DOI: https://doi.org/10.1038/s41598-026-43201-8