Fig. 1 | Scientific Reports

Fig. 1

From: VS-4718 enhances apoptosis induced by low-dose carfilzomib and overcomes carfilzomib resistance in PSMB5-mutated proteasome inhibitor resistant multiple myeloma

Fig. 1

Impact of VS-4718 on viability and PARP-1 cleavage in seven MM cell lines. (A) MM cells were treated with different concentrations of VS-4718 (0.1–10 µM) for 96 h and viability subsequently measured by MTT assay in three independent experiments. (B, C) MM cells were treated with 2 µM VS-4718 and PARP-1 cleavage determined by Western blot analysis in three independent experiments (R1, R2, R3). (B) Only one representative GAPDH (R2) was shown in this Western blot picture. (C) Image J analysis was performed to analyze the GAPDH-normalized expression levels of cleaved PARP-1 compared to the DMSO control. Bars represent the mean ± SEM of three independent experiments. Western blots were cropped. For uncropped Western blots see supplementary information.

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