Fig. 5 | Scientific Reports

Fig. 5

From: VS-4718 enhances apoptosis induced by low-dose carfilzomib and overcomes carfilzomib resistance in PSMB5-mutated proteasome inhibitor resistant multiple myeloma

Fig. 5

Western blot analysis to determine the PYK2 and FAK expression and activation status before and after inhibition with VS-4718 and carf alone and in combination. (A) MM.1 S cells treated with 3 µM VS-4718 and 1.5 nM carf alone and MM1.SR180Ixa cells with 3 µM VS-4718 and 2 nM carf for 24 h (B) L-363 cells were treated with 3 µM VS-4718 and 3 nM carf and L363R350Ixa cells with 3 µM VS-4718 and 6 nM carf for 24 h. (A, B) Image J analysis was performed to analyze the GAPDH-normalized expression and activation levels of (p)PYK2 and (p)FAK compared to the DMSO control. Bars represent the mean ± SD of three independent experiments. Western blots were cropped. For uncropped Western blots see supplementary information. P-values were calculated with a two-way ANOVA and an ordinary one-way ANOVA (for FAK in L-363 and L-363R350Ixa) using the Tukey’s multiple comparison test with multiple comparison test and only depicted for p < 0.05.

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