Fig. 4
B. bavariensis NT24 and JHM1114 resist complement-mediated killing in human serum. The percentage of motile cells of B. bavariensis NT24, JHM1114, PBi, and B. garinii G1 grown in either 50% NHS (a) or 50% hiNHS (b) were determined by dark-field microscopy at 0, 2, 4, and 6 h. At least three independent biological replicates were assayed for NHS-treated spirochetes and two independent biological replicates were conducted for hiNHS-treated cells. Each test gave similar results. For clarity, only data from a representative experiment is shown. Error bars indicate standard deviation (SD). For all plots, abbreviations refer to: NHS, non-immune human serum, hiNHS, heat-inactivated NHS.
