Fig. 1
Expression of mouse beta-defensin-1 (mBD1) in the murine experimental biliary atresia. (A) Expression (copies/10 ng RNA normalized to GAPDH) of mBD1 in the normal neonatal murine liver compared to intestinal and bile duct tissue as measured by qRT-PCR (n = 24 mice, normal mouse cohort). Significance symbols indicate pairwise comparisons of liver vs. each indicated tissue based on Tukey-adjusted p values. (B) Liver expression of mBD1 in an RRV model of experimental BA in Balb/c compared to mice that did not receive i.p. RRV injection and did not develop BA (n = 4), mice receiving RRV injection but not developing BA (n = 10), and mice receiving an RRV injection and developing BA (n = 10). Significance symbols indicate pairwise comparisons of RRV receiving mice groups versus non-RRV mice. (C) Bile duct expression of mBD1 in the mouse cohort from (B). Significance symbols indicate pairwise comparisons of RRV receiving mice groups versus non-RRV mice. Means and 95% confidence intervals are shown. Group differences were analyzed by one-way ANOVA with Tukey’s multiple comparisons test (ns, p > 0.05 ; *, p ≤ 0.05; ****, p ≤ 0.0001). Abbreviations: BA, biliary atresia; RRV, Rhesus Rotavirus; RT-PCR, real-time polymerase chain reaction.
