Table 5 Experimental jetting parameters set at the SPB/SFX interaction region upstream (IRU) sample chamber (p\(\approx\)\(10^{-3}\) mbar), X-ray parameters and jet characterization results. The ’effective pulse energy’ considers the measured pulse energy at the XTD2 X-ray gas monitor, the selected attenuators at the XTD9 tunnel (total transmission) and that the transmission with the nano-KB mirrors is about 60%. The results from the ’scaling law’ are obtained by applying the jet velocity prediction formula from2. Jets with velocities of v\(\ge\)25 m/s are fast enough for 0.564 MHz operation, while 1.13 MHz operation is compatible with jets with v\(\ge\)45 m/s.
Sample flow | Sheath flow | Total Q | Dilution | He flow | X-ray parameters | Jet explosion-based characterization | Scaling law | Microscopy | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample | Flow rate [μL/min] | Solution | Flow rate [μL/min] | [μL/min] | x-Fold | Flow rate [mg/min] | Intra-train rep. rate [Mega 1/s] | No. pulses/train | Photon energy [keV] | Pulse energy [mJ] | Transm. [%] | Eff. pulse energy [μJ] | Detector distance [mm] | Displacement between two pulses [μm] | Jet velocity [m/s] | Jet diameter calc. [μm] | Jet velocity theo. [m/s] | Jet diameter theo. [μm] | Jet diameter [μm] |
HEWL | 30.0 | NA | 0.0 | 30.0 | 1.00 | 27.0 | 0.564 | 202 | 11.0 | 1.49 | 2 | 18 | 124.3 | 79.3 | 44.7 | 3.77 | 46.2 | 3.71 | 4.28±0.87 |
HEWL | 20.0 | Water | 10.0 | 30.0 | 1.50 | 31.0 | 1.128 | 100 | 11.0 | 0.83 | 47 | 234 | 124.3 | 41.6 | 46.9 | 3.68 | 48.9 | 3.61 | 3.68±0.38 |
HEWL | 20.0 | PEO 1% | 10.0 | 30.0 | 1.50 | 28.5 | 1.128 | 352 | 11.0 | 0.75 | 47 | 212 | 124.3 | 38.5 | 43.5 | 3.83 | 47.2 | 3.67 | 4.12±0.19 |
HEWL | 10.0 | PEO 1% | 20.0 | 30.0 | 3.00 | 28.4 | 1.128 | 352 | 11.0 | 0.74 | 47 | 209 | 124.3 | 35.8 | 40.3 | 3.97 | 47.1 | 3.68 | 4.12±0.38 |
HEWL2 | 5.0 | PEO 1% | 12.5 | 17.5 | 3.50 | 20.9 | 1.128 | 352 | 11.0 | 0.89 | 22 | 117 | 124.3 | 39.5 | 44.6 | 2.89 | 46.4 | 2.83 | 3.47±0.38 |
HEWL2 | 10.0 | PEO 1% | 12.5 | 22.5 | 2.25 | 23.5 | 1.128 | 352 | 11.0 | 0.91 | 22 | 120 | 124.3 | 37.7 | 42.5 | 3.35 | 46.3 | 3.21 | 3.68±0.38 |
HEWL2 | 20.0 | PEO 1% | 12.5 | 32.5 | 1.63 | 29.8 | 1.128 | 352 | 11.0 | 0.88 | 22 | 116 | 124.3 | 38.4 | 43.3 | 3.99 | 47.3 | 3.82 | 4.33±0.75 |
PSII | 20.0 | PEO 1% | 10.0 | 30.0 | 1.50 | 19.9 | 0.564 | 202 | 12.5 | 1.10 | 43 | 284 | 126.6 | 57.9 | 32.7 | 4.42 | 40.8 | 3.95 | 4.41±0.63 |
PSII | 25.0 | EtOH | 40.0 | 65.0 | 2.60 | 30.0 | 0.564 | 202 | 9.3 | 1.75 | 100 | 1050 | 166.0 | 78.1 | 44.0 | 5.60 | 41.2 | 5.79 | 5.21±0.19 |
PSI | 20.0 | EtOH | 10.0 | 30.0 | 1.50 | 33.0 | 1.128 | 352 | 9.3 | 2.60 | 40 | 624 | 168.2 | 39.2 | 44.2 | 3.79 | 44.2 | 3.80 | 3.02±0.45 |
PSI | 30.0 | NA | 0.0 | 30.0 | 1.00 | 33.0 | 1.128 | 352 | 9.3 | 2.57 | 40 | 617 | 168.2 | 40.1 | 45.2 | 3.75 | 44.2 | 3.80 | 2.81±0.32 |
