Fig. 6
From: IFITM3-mediated neuroinflammation in epilepsy regulated by the let-7g-5p/STAT2 axis
STAT2 promotes inflammation, apoptosis, and pyroptosis through modulation of IFITM3. (A–C) Levels of IL-1β, IL-6, and TNF-α were quantified in BV2 cells transfected with STAT2, si-IFITM3-3, or the corresponding negative controls. The rescue experiments involved co-transfection of STAT2 with si-IFITM3-3. (D–F) The expression levels of IL-1β, IL-6, and TNF-α in BV2 cells transfected with si-STAT2-3, IFITM3, or the respective negative controls. The rescue experiments involved co-transfection of si-STAT2-3 with IFITM3. (G) The apoptosis rate of BV2 cells transfected with si-STAT2-3, IFITM3, or their negative controls. (H) Flow cytometry was performed to assess apoptosis rate in each treatment group. The expression levels of (I,K) ASC and (J,L) IL-18 in BV2 cells treated with different plasmids were detected by RT-qPCR analysis.
