Fig. 7

STAT2 as a potential target of let-7g-5p. (A) Let-7e-5p and (B) let-7g-5p were significantly downregulated in LPS-stimulated BV2 cells. (C,D) The relative overexpression efficiency of let-7e-5p mimic and let-7g-5p mimic was examined by RT-qPCR. (E,F) The relative knockdown efficiency of let-7e-5p inhibitor and let-7g-5p inhibitor was assessed using RT-qPCR. (G,H) STAT2 expression levels in BV2 cells transfected with let-7e-5p mimic or let-7g-5p mimic were measured by RT-qPCR. (I,J) STAT2 expression levels in BV2 cells transfected with let-7e-5p inhibitor or let-7g-5p inhibitor were evaluated by RT-qPCR. (K) Relative luciferase activities in HEK293T cells co-transfected with STAT2-3′UTR together with let-7e-5p mimic, let-7g-5p mimic, or negative controls. (L) A schematic diagram of the bases targeted by let-7g-5p in the STAT2 3′-UTR, as well as the base sequence of the mutated STAT2 3′-UTR. (M) Relative luciferase activities of STAT2-3′UTR and STAT2-3′UTR-mut co-transfected with let-7g-5p mimic or a negative control. Let-7g-5p expression in the cortex (N) and hippocampus (O) of epileptic mice was assessed by RT-qPCR (n = 16).