Abstract
Coriander (Coriandrum sativum L.), a widely cultivated herb valued for its culinary and medicinal uses, is increasingly threatened by soil-borne fungal pathogens. During a field survey in Al-Beqai’a, Tubas district (Palestine) in spring 2024, coriander plants exhibited symptoms of yellowing, wilting, and damping-off. Morphological examination and molecular identification using ITS1 and ITS4 primers confirmed Fusarium foetens as the causal agent, marking the first report of this pathogen on coriander in Palestine. Pathogenicity was validated through inoculation trials fulfilling Koch’s postulates. Given the limitations of chemical fungicides, twelve essential oils (EOs) were evaluated for antifungal activity against F. foetens. Disc diffusion and minimum inhibitory concentration (MIC) assays revealed that wild thyme (Thymus serpyllum) and pine (Pinus sylvestris) EOs exhibited the most potent inhibitory effects, both having MIC values of 25 µg/mL. The antifungal efficacy of these EOs is likely attributed to their phenolic and terpene-rich composition, offering a promising, eco-friendly alternative for disease management. This study underscores the emergence of F. foetens as a phytopathogen of coriander and highlights the potential of EOs in integrated fungal disease control strategies.
Data availability
Data will be available upon request. The datasets generated and/or analyzed during the current study are available in the [NCBI] repository under the accession number “PV864959” [https://www.ncbi.nlm.nih.gov/nuccore/PV864959].
References
Srinivasan, K. Coriander (Coriandrum sativum L.) - A potential source of bioactive compounds for food and medicinal applications. Food Rev. Int. 22, 173–198 (2006).
Petropoulos, S. A., Fernandes, Â., Barros, L. & Ferreira, I. C. F. R. Chemical composition, nutritional value and antioxidant properties of Mediterranean herbs and spices: A review. Food Chem. 327, 127145 (2020).
Sahib, A. S., Salman, H. A. & Anwar, M. A. Antioxidant activity of coriander (Coriandrum sativum L.) essential oil. J. Pharm. Sci. Res. 5, 136–139 (2013).
Dean, R. et al. The top 10 fungal pathogens in molecular plant pathology. Mol. Plant Pathol. 13, 414–430 (2012).
Summerell, B. A. Resolving Fusarium: Current status of the genus. Annu. Rev. Phytopathol. 57, 323–339 (2019).
Haware, M. P. & Nene, Y. L. Screening of germplasm for resistance to wilt. Int. Chickpea Newsl. 6, 13–14 (1982).
Al-Samarrai, G., Singh, H. & Syarhabil, M. Investigating the antifungal activity of aqueous extracts of some medicinal plants on Fusarium oxysporum. Afr. J. Biotechnol. 11, 170–175 (2012).
Sharma, A., Sharma, P., Raj, D. & Sharma, P. N. Wilt of coriander (Coriandrum sativum) incited by Fusarium oxysporum – A new record from India. Arch. Phytopathol. Plant Prot. 47, 1059–1064 (2014).
Bakkali, F., Averbeck, S., Averbeck, D. & Idaomar, M. Biological effects of essential oils: A review. Food Chem. Toxicol. 46, 446–475 (2008).
Burt, S. Essential oils: Their antibacterial properties and potential applications in foods; a review. Int. J. Food Microbiol. 94, 223–253 (2004).
Dorman, H. J. D. & Deans, S. G. Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. J. Appl. Microbiol. 88, 308–316 (2000).
Pinto, E., Vale-Silva, L., Cavaleiro, C. & Salgueiro, L. Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species. J. Med. Microbiol. 58, 1454–1462 (2009).
Hyldgaard, M., Mygind, T. & Meyer, R. L. Essential oils in food preservation: Mode of action, synergies, and interactions with food matrix components. Front. Microbiol. 3, 12 (2012).
Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R. & De Feo, V. Effect of essential oils on pathogenic bacteria. Pharmaceuticals 6, 1451–1474 (2013).
Leslie, J. F. & Summerell, B. A. The Fusarium Laboratory Manual (Blackwell Publishing, 2006).
Barnett, H. L. & Hunter, B. B. Illustrated Genera of Imperfect Fungi (APS, 1998).
Samson, R. A., Houbraken, J., Thrane, U., Frisvad, J. C. & Andersen, B. Food and Indoor Fungi (CBS-KNAW Fungal Biodiversity Centre, 2010).
Kirk, P. M., Cannon, P. F., Minter, D. W. & Stalpers, J. A. Ainsworth & Bisby’s Dictionary of the Fungi (CAB International, 2008).
White, T. J., Bruns, T., Lee, S. & Taylor, J. W. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: (eds Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J.) PCR Protocols: A Guide to Methods and Applications. Academic, 315–322 (1990).
Kumar, S. et al. MEGA12: Molecular evolutionary genetic analysis version 12 for adaptive and green computing. Mol. Biol. Evol. 41, msae263 (2024).
Jaradat, N. A., Zaid, A. N., Abuzant, A. & Shawahna, R. Investigation the efficiency of various methods of volatile oil extraction from Trichodesma africanum and their impact on the antioxidant and antimicrobial activities. J. Intercult. Ethnopharmacol. 5, 250–256 (2016).
Jaradat, N. Qualitative and quantitative comparison of aromatic oil components and antifungal effects of Cymbopogon flexuosus obtained with supercritical CO₂, microwave–ultrasonic, steam distillation, and hydrodistillation extraction techniques. Molecules 28, 6870 (2023).
Eid, A. M. & Jaradat, N. Public knowledge, attitude, and practice on herbal remedies used during pregnancy and lactation in West Bank Palestine. Front. Pharmacol. 11, 46 (2020).
Balouiri, M., Sadiki, M. & Ibnsouda, S. K. Methods for in vitro evaluating antimicrobial activity: A review. J. Pharm. Anal. 6, 71–79 (2016).
Wiegand, I., Hilpert, K. & Hancock, R. E. W. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Protoc. 3, 163–175 (2008).
Chaimovitsh, D. et al. Fusarium wilt of coriander: Root cause analysis and varietal tolerance development. Plants 13, 2135 (2024).
Summerell, B. A., Salleh, B. & Leslie, J. F. A utilitarian approach to Fusarium identification. Plant Dis. 94, 964–972 (2010).
Domsch, K. H., Gams, W. & Anderson, T. H. Compendium of Soil Fungi (IHW-, 2007).
Abdel-Kader, M. M., El-Mougy, N. S. & Lashin, S. M. Integration of some natural products with soil solarization for controlling soilborne pathogens in cucumber. Arch. Phytopathol. Plant Prot. 45, 1034–1046 (2012).
Lombard, L., van Dam, P., Figueiredo, L. N., Crous, P. W. & Wingfield, M. J. Fusarium species: Molecular tools for species identification and taxonomy. Plant Pathol. 68, 1013–1029 (2019).
Hall, B. G. Building phylogenetic trees from molecular data with MEGA. Mol. Biol. Evol. 30, 1229–1235 (2013).
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35, 1547–1549 (2018).
Bayraktar, H. & Dolar, F. S. Molecular identification and genetic diversity of Fusarium species associated with onion fields in Turkey. J. Phytopathol. 160, 297–302 (2012).
Schroers, H. J. et al. Taxonomy and phylogeny of Fusarium species causing basal rot of begonia and impatiens. Mycologia 101, 499–510 (2009).
Blok, W. J., Van der Werf, D. C. & Termorshuizen, A. J. Fusarium foetens, a new species causing stem rot in begonia. Mycologia 100, 545–554 (2008).
Garibaldi, A., Gilardi, G. & Gullino, M. L. First report of Fusarium foetens causing basal stem rot on lettuce in Italy. Plant Dis. 94, 376 (2010).
Zhang, Y. et al. The fungicidal activity of thymol against Fusarium graminearum via inducing lipid peroxidation and disrupting ergosterol biosynthesis. Molecules 21, 770 (2016).
Saghrouchni, H. et al. Potential of carvacrol as plant growth-promoter and green fungicide against Fusarium wilt disease of perennial ryegrass. Agriculture 13, 189 (2023).
de Barros, D. B., Gontijo, J. R. & Silva, L. A. α-Pinene: Docking study, cytotoxicity, mechanism of action, and anti-biofilm effect against Candida albicans. Antibiotics 12, 480 (2022).
Isman, M. B. Plant essential oils for pest and disease management. Crop Prot. 19, 603–608 (2000).
Google Maps. Tammun – Tubas Governorate. Retrieved 29 June (2025).
Acknowledgment
The authors gratefully acknowledge An-Najah National University and the Ministry of Agriculture for their support and assistance in facilitating the laboratory work. Authors appreciate the efforts of Dr. Abdurrahman Eid for his valuable comments and revision of statistical analysis.
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R.A. and N.J. wrote the manuscript and designed research work. R.K. and E.E. did the isolation and identification of the pathogen. A.A.; I.A.I.; A.S.I. and A.H. did molecular tests; S.M.; L.G.; M.H.H.; N.M.; and H.S. did antifungal tests.
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Alkowni, R., Jaradat, N., Kmail, R. et al. First Isolation of Fusarium foetens from coriander in Palestine and preliminary evaluation of essential oils for its control. Sci Rep (2026). https://doi.org/10.1038/s41598-026-44502-8
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DOI: https://doi.org/10.1038/s41598-026-44502-8
