Table 1 Analysis of laboratory-prepared mixtures of SOF and MIR using different divisors by the proposed spectrophotometric ratio method.
No. | Concentration (µg/mL) | Method | Constant center method | Unified constant subtraction | ||||||||
divisor | Normalized divisor | MIR 3.00 µg/mL | MIR 8.00 µg/mL | MIR 14.00 µg/mL | Extracted MIR | |||||||
Ratio | SOF | MIR | SOF | MIR | SOF | MIR | SOF | MIR | SOF | MIR | ||
SOF: MIR | ||||||||||||
1 | 2.8:14.0a | 1:5 | 98.73 | 98.02 | 112.56 | 99.35 | 97.58 | 97.82 | 99.88 | 99.20 | 99.73 | 99.74 |
2 | 5.6:14.0 | 2:5 | 89.69 | 101.61 | 124.83 | 100.58 | 101.79 | 98.84 | 98.91 | 99.61 | 99.48 | 99.15 |
3 | 7.0:7.0 | 1:1 | 97.10 | 100.53 | 112.30 | 101.97 | 104.47 | 100.12 | 99.56 | 99.66 | 99.86 | 100.15 |
4 | 14.0:7.0 | 2:1 | 97.86 | 99.71 | 106.84 | 101.35 | 101.77 | 99.51 | 99.86 | 99.05 | 99.93 | 100.17 |
5 | 3.5:14.0 | 1:4 | 88.20 | 100.58 | 138.89 | 102.12 | 107.56 | 100.38 | 99.42 | 99.75 | 100.11 | 100.30 |
Laboratory Prepared mixturesMean % b± SD | 94.32± 4.96 | 100.09± 1.34 | 119.09 ±12.88 | 101.00 ±1.14 | 102.63± 3.69 | 99.33 ±1.04 | 99.52±0.40 | 99.46± 0.31 | 99.82 ±0.24 | 99.92 ± 0.47 | ||
Average Bias %c | 5.68 | 0.09 | 19.09 | 1.00 | 2.63 | 0.67 | 0.48 | 0.54 | 0.18 | 0.08 | ||
Average RSD% d | 5.26 | 0.74 | 10.82 | 1.12 | 3.60 | 1.24 | 0.40 | 0.69 | 0.37 | 0.32 | ||
Robustness (±0.1 nm ) RSD % e | 6.40 | 1.16 | 9.23 | 0.92 | 2.91 | 1.01 | 1.00 | 0.75 | 0.39 | 0.36 | ||
Cumulative Validation Score (CVS) f | 17.34 | 1.99 | 39.14 | 3.04 | 9.14 | 2.92 | 1.88 | 1.98 | 0.94 | 0.76 | ||
