Fig. 1 | Scientific Reports

Fig. 1

From: Real-time bioluminescence imaging of mycobacteria with Akaluc: a novel method for monitoring drug efficacy

Fig. 1The alternative text for this image may have been generated using AI.

Comparison of substrates, concentration and determining optimum in vitro imaging time. Level of bioluminescence response of three different substrates in four different concentrations at four different time points was compared in interaction with BCGT1::pMV261::PrAg85B::Akaluc. (a) Comparison of the concentrations yielding the strongest bioluminescence for TokeOni, SeMpai, and CycLuc 1 at each measurement point. (b) Comparison among timepoints with fixed substrate concentration (c) Comparison among different substrate concentrations of the same substrate at a specific time point. Two-way ANOVA followed by Tukey’s multiple comparisons test was used to test for significance between substrates, concentrations and time points. Data represent means ± s.e.m. ∗  ∗  ∗  ∗ P< 0.0001, ∗  ∗  ∗ P < 0.001, ∗  ∗ P < 0.01 ns, no significance.

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