Fig. 2
In vitro kinetics of M. smegmatis-Akaluc. M. smegmatis mc2 155 transformed with 4 different bioluminescent reporter plasmids ((pSO246::PrAg85B::Akaluc, pSO246::PrACR::Akaluc, pMV261::PrAg85B::Akaluc and pMV261::PrACR::Akaluc), and 2 empty vectors (pSO246 and pMV261) and their growth kinetics along with bioluminescence was evaluated under normal oxygen. After adjusting OD600 at 0 h, all parameters (OD600, CFU, and bioluminescence) were measured at 12 h and followed to 48 h at an interval of 6 h. (a) Time versus bacterial density (OD600), (b) Time versus bioluminescence, (c) Time versus Log10 CFU, (d) Bacterial density (OD600) versus bioluminescence. Two-way ANOVA followed by Tukey’s multiple comparisons test was used to test for significance between groups for OD600, CFU, and Bioluminescence. Data represent means ± s.e.m. from three replicates (n = 3), # P < 0.0001, hash symbols (#) are inserted where pMV261::PrAg85B::Akaluc significantly (P < 0.05) differed with all other groups.
