Fig. 2
Peripheral and central guttae in cross-sections. (A) Periodic acid-Schiff (PAS) staining of a corneal cross-section, highlighting Descemet membrane (DM) with cross-sectioned guttae. The central DM is overlaid with a posterior fibrillar layer (PFL, blue arrows), which both surrounds and covers the guttae (red arrows). Refer to suppl. Fig. S3 for images of the whole cornea. (B) Green autofluorescence observed in unstained paraffin sections of a cornea. Autofluorescence is prominently localized within the DM and extends into the guttae (red arrows). While the PFL (yellow arrows) was detectable on the posterior layer overlaying a central gutta using DIC imaging, it did not exhibit green autofluorescence. The corneal epithelium is shown for comparison. It does not show a PFL and has much weaker autofluorescence. B indicates Bowman’s layer (anterior limiting membrane).
